CJC-1295 (without DAC / Mod GRF 1-29) vs TB-500 (Thymosin Beta-4)

Side-by-side comparison of mechanisms, dosing, interactions, and stacking potential.

CJC-1295 (without DAC / Mod GRF 1-29)TB-500 (Thymosin Beta-4)
CategoryPeptidesPeptides
Standard DoseResearch indicates 100-300 mcg administered 1-3 times daily via subcutaneous injection.Research indicates 2-2.5 mg administered twice weekly via subcutaneous injection.
TimingBest administered at bedtime (primary), upon waking, and/or post-workout. Always on empty stomach — wait 2+ hours after last meal. Avoid carbohydrates 30+ minutes after injection.No strict timing requirements. Can be administered at any time of day. Systemic action means injection location is not critical.
Cycle Duration12-24 week cycles with 4-8 week breaks.Loading phase: 4-6 weeks. Total cycle: 8-16 weeks.
Evidence Levelmoderate_humananimal_plus_anecdotal

Mechanism

Mod GRF 1-29 (Modified Growth Hormone Releasing Factor, amino acids 1-29) is a truncated and modified GHRH analog that binds to GHRH receptors on anterior pituitary somatotrophs, activating cAMP via Gs protein/adenylate cyclase and mitogen-activated protein kinase pathways. Without the DAC moiety, it has a short half-life of approximately 30 minutes, producing acute GH pulses that more closely mimic natural pulsatile GH secretion. It also stimulates pituitary gene transcription of GH mRNA, preserving endogenous pituitary reserve.

Standard Dosing

Research indicates 100-300 mcg administered 1-3 times daily via subcutaneous injection.

Timing

Best administered at bedtime (primary), upon waking, and/or post-workout. Always on empty stomach — wait 2+ hours after last meal. Avoid carbohydrates 30+ minutes after injection.

Cycle Duration

12-24 week cycles with 4-8 week breaks.

Side Effects

  • Flushing post-injection
  • Head rush
  • Hunger increase
  • Water retention (mild)
  • Tingling in extremities

Contraindications

  • Active cancer
  • Diabetic retinopathy
  • Pregnancy and breastfeeding
  • Intracranial hypertension

Best Stacking Partners

IpamorelinGHRP-2GHRP-6Hexarelin

Mechanism

TB-500 is a synthetic fragment of Thymosin Beta-4, a 43-amino acid protein that sequesters G-actin monomers, preventing premature polymerization and facilitating cellular migration and morphological changes essential for wound healing. It upregulates actin to promote cell migration, proliferation, and differentiation of stem/progenitor cells at injury sites. TB-500 also enhances angiogenesis, reduces inflammation, and promotes tissue remodeling through increased re-epithelialization and vascular density.

Standard Dosing

Research indicates 2-2.5 mg administered twice weekly via subcutaneous injection.

Timing

No strict timing requirements. Can be administered at any time of day. Systemic action means injection location is not critical.

Cycle Duration

Loading phase: 4-6 weeks. Total cycle: 8-16 weeks.

Side Effects

  • Temporary lethargy
  • Head rush upon injection
  • Injection site irritation
  • Mild headache

Contraindications

  • Active cancer (angiogenesis concern)
  • Pregnancy and breastfeeding
  • Recent myocardial infarction

Best Stacking Partners

BPC-157GHK-CuGH Secretagogues

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